Comparison of Various Control Techniques for Clove Stem Borer (Nothopeus hemipterus) in Samigaluh, Kulon Progo
DOI:
https://doi.org/10.25077/jrp.6.1.25-35.2025Keywords:
Beauveria bassiana, Citronella Oil, Clove, Pesticidem, Stem BorerAbstract
The clove stem borer (Nothopeus hemipterus) is a major pest causing significant economic losses in clove cultivation. In Samigaluh, most farmers rely on chemical pesticides for control. However, essential oils and entomopathogenic fungi offer safer and more sustainable alternatives. This study aimed to evaluate their effectiveness and determine the most suitable control method. The research was conducted in Kayugedhe Hamlet, Samigaluh, Kulon Progo, Yogyakarta, from April to September 2022. A randomized completely block design was used with different pest control treatments, including chemical pesticides (fipronil, acephate, and profenofos), botanical pesticides (10% neem oil and 10% citronella oil), biological pesticides (Beauveria bassiana at 1 × 10⁸ CFU/mL), and a control. The pesticides were applied using a hydraulic pump and a wax-sealing method on larval entry holes. Larval mortality was recorded weekly for four weeks. The results showed that the application of entomopathogenic fungi (Beauveria bassiana) and citronella oil led to larval mortality rates of 78% and 75%, respectively, which were comparable to the effectiveness of fipronil (80%) and acephate (81%). In contrast, N. hemipterus larvae exhibited lower susceptibility to profenofos, with a mortality rate of only 47%, suggesting potential resistance. These findings indicate that B. bassiana and citronella oil extract could serve as effective and environmentally friendly alternatives for controlling the clove stem borer.
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References
Abdel-Hakim, E. A., Ibrahim, S. S., & Salem, N. Y. (2021). Effect of garlic and lemongrass essential oils on some biological and biochemical aspects of corn stem borer Sesamia cretica larvae (Lepidoptera: Noctuidae) during diapausing phase. Proceedings of the Zoological Society, 74(1), 73–82. https://doi.org/10.1007/s12595-020-00348-0
Altinok, H. H., Altinok, M. A., & Koca, A. S. (2019). Modes of action of entomopathogenic fungi. Current Trends in Natural Sciences, 8(16), 117–124.
Alves, M. D. S., Campos, I. M., Brito, D. D. M. C. D., Cardoso, C. M., Pontes, E. G., & Souza, M. A. A. D. (2019). Efficacy of lemongrass essential oil and citral in controlling Callosobruchus maculatus (Coleoptera: Chrysomelidae), a post-harvest cowpea insect pest. Crop Protection, 119, 191–196. https://doi.org/10.1016/j.cropro.2019.02.007
Amoabeng, B. W., Johnson, A. C., & Gurr, G. M. (2019). Natural enemy enhancement and botanical insecticide source: A review of dual use companion plants. Applied Entomology and Zoology, 54(1), 1–19. https://doi.org/10.1007/s13355-018-00602-0
Andriani, A. A. S. P. R., Wirajaya, A. A. N. M., Mahardika, I. B. K., Kartini, L., Arjana, I. G. M., Situmeang, Y. P., Suaria, I. N., Yuliartini, M. S., Sudewa, K. A., Astiari, N. K. A., Udayana, I. G. B., & Pakusi, S. (2023). Control of clove plant disturbing organisms in amerta masa farmer group in Asahduren Village, Pekutatan District, Jembrana Regency. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment). https://doi.org/10.29165/ajarcde.v7i3.326
Astuti, Y., & Maryani, Y. (2016). Hama dan Penyakit Utama pada Tanaman Cengkeh. Direktorat Perlindungan Tanaman Perkebunan.
Avianto, Y., Noviyanto, A., Sidiq, M. F., Hernowo, & Pratama, A. B. (2024). Reforestation Impact: Contrasting Carbon Stock and Biodiversity of Clove in Monoculture and Mixed Gardens Over 25 Years. Jurnal Biologi Tropis, 24(4), 333–340. https://doi.org/10.29303/jbt.v24i3.7253
Ávila-Hernández, J. G., Aguilar-Zárate, P., Carrillo-Inungaray, M. L., Michel, M. R., Wong-Paz, J. E., Muñiz-Márquez, D. B., Rojas-Molina, R., Ascacio-Valdés, J. A., & Martínez-Ávila, G. C. G. (2022). The secondary metabolites from Beauveria bassiana PQ2 inhibit the growth and spore germination of Gibberella moniliformis LIA. Brazilian Journal of Microbiology, 53(1), 143–152. https://doi.org/10.1007/s42770-021-00668-z
Baek, S., Noh, M. Y., Mun, S., Lee, S. J., Arakane, Y., & Kim, J. S. (2022). Ultrastructural analysis of beetle larva cuticles during infection with the entomopathogenic fungus, Beauveria bassiana. Pest Management Science, 78(8), 3356–3364. https://doi.org/10.1002/ps.6962
Benelli, G., Canale, A., Toniolo, C., Higuchi, A., Murugan, K., Pavela, R., & Nicoletti, M. (2017). Neem ( Azadirachta indica ): Towards the ideal insecticide? Natural Product Research, 31(4), 369–386. https://doi.org/10.1080/14786419.2016.1214834
Boaventura, D., Bolzan, A., Padovez, F. E., Okuma, D. M., Omoto, C., & Nauen, R. (2020). Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda. Pest Management Science, 76(1), 47–54. https://doi.org/10.1002/ps.5505
Dannon, H. F., Dannon, A. E., Douro-Kpindou, O. K., Zinsou, A. V., Houndete, A. T., Toffa-Mehinto, J., Elegbede, I. A. T. M., Olou, B. D., & Tamò, M. (2020). Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. Journal of Cotton Research, 3(1), 24. https://doi.org/10.1186/s42397-020-00061-5
Danthu, P., Simanjuntak, R., Fawbush, F., Leong Pock Tsy, J. M., Razafimamonjison, G., Abdillahi, M. M., Jahiel, M., & Penot, E. (2020). The clove tree and its products (clove bud, clove oil, eugenol): Prosperous today but what of tomorrow’s restrictions? Fruits, 75(5), 224–242. https://doi.org/10.17660/th2020/75.5.5
Fiana, Y., Nurbani, & Danial, D. (2015, August 1). Kajian keefektifan agen hayati Beauveria bassiana dan penyarungan buah dalam pengendalian hama PBK di Kalimantan Timur. Seminar Nasional Masyarakat Biodiversitas Indonesia. https://doi.org/10.13057/psnmbi/m010545
Islam, S. M. N., Chowdhury, Md. Z. H., Mim, M. F., Momtaz, M. B., & Islam, T. (2023). Biocontrol potential of native isolates of Beauveria bassiana against cotton leafworm Spodoptera litura (Fabricius). Scientific Reports, 13(1), 8331. https://doi.org/10.1038/s41598-023-35415-x
Juma, P., Njau, N., W., F. W., Micheni, C. M., Khan, H. A., Mitalo, O. W., & Odongo, D. (2022). Trends in Neem (Azadirachta indica)-Based Botanical Pesticides. In S. D. Mandal, G. Ramkumar, S. Karthi, & F. Jin (Eds.), New and Future Development in Biopesticide Research: Biotechnological Exploration (pp. 137–156). Springer Nature Singapore. https://doi.org/10.1007/978-981-16-3989-0_5
Keswani, C., Singh, S. P., & Singh, H. B. (2013). Beauveria bassiana: Status, mode of action, applications and safety issues. Biotech Today, 3(1), 16. https://doi.org/10.5958/j.2322-0996.3.1.002
Kulendeng, J., Basir, M., & Asrul. (2021). Kerusakan pohon cengkeh akibat serangan hama penggerek batang (Nothopeus hemipterus) di Kecamatan Peling Tengah Kabupaten Banggai Kepulauan. Mitra Sains, 9(2), 71–79. https://doi.org/10.22487/ms26866579.2021.v9.i2.pp71-79
Luanmasa, S. P., Leatemia, J. A., & Uruilal, C. (2023). Intensitas kerusakan tanaman cengkeh (Syzygium aromaticum L.) akibat serangan hama dan penyakit di Negeri Hitu Lama, Kecamatan Leihitu Kabupaten Maluku Tengah. Jurnal Agrosilvopasture-Tech, 2(2), 412–420. https://doi.org/10.30598/j.agrosilvopasture-tech.2023.2.2.412
Manikandan, K. R., Muthuswani, M., Chitra, N., & Ananthan, M. (2019). Management of coffee white stem borer Xylotrechus quadripes (Chevrolat, 1863) (Coleoptera: Cerambycidae) in the Lower Pulney Hills, India. Int.J.Curr.Microbiol.App.Sci, 8(6), 1–11.
Mejía, C., Espinel, C., Forero, M., Ramos, F. A., Brandão, P. F. B., & Villamizar, L. (2020). Improving ecological fitness of Beauveria bassiana conidia to control the sugar cane borer Diatraea saccharalis. Biocontrol Science and Technology, 30(6), 513–530. https://doi.org/10.1080/09583157.2020.1738343
Moustafa, M. A. M., Ahmed, F. S., Alfuhaid, N. A., El-Said, N. A., Ibrahim, E.-D. S., & Awad, M. (2024). The Synergistic Effect of Lemongrass Essential Oil and Flometoquin, Flonicamid, and Sulfoxaflor on Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae): Insights into Toxicity, Biochemical Impact, and Molecular Docking. Insects, 15(5), 302. https://doi.org/10.3390/insects15050302
Moustafa, M., Awad, M., Amer, A., Hassan, N., Ibrahim, E.-D., Ali, H., Akrami, M., & Salem, M. (2021). Insecticidal activity of lemongrass essential oil as an eco-friendly agent against the black cutworm Agrotis ipsilon (Lepidoptera: Noctuidae). Insects, 12(8), 737. https://doi.org/10.3390/insects12080737
Otunola, G. A. (2022). Culinary spices in food and medicine: An Overview of Syzygium aromaticum (L.) Merr. and L. M. Perry [Myrtaceae]. Frontiers in Pharmacology, 12, 793200. https://doi.org/10.3389/fphar.2021.793200
Rimbing, J., Engka, R., Kandowangko, D., & Rorong, F. (2019). The Use of Insecticides to Protect Cocoa Fruit from the Attack of Cocoa Pod Borer, Conopomorpha cramerella in Cocoa Plants. International Journal of ChemTech Research, 12(04), 226–236. https://doi.org/10.20902/IJCTR.2019.120428
Riyaz, M., Mathew, P., Zuber, S. M., & Rather, G. A. (2022). Botanical Pesticides for an Eco-Friendly and Sustainable Agriculture: New Challenges and Prospects. In S. A. Bandh (Ed.), Sustainable Agriculture (pp. 69–96). Springer International Publishing. https://doi.org/10.1007/978-3-030-83066-3_5
Rizal, M. (2017). Pengendalian Terpadu Hama Penggerek Batang Cengkeh. Balai Penelitian Tanaman Rempah dan Obat.
Runaweri, C., Pelealu, J., & Manueke, J. (2017). Serangan dan kerusakan tanaman cengkeh yang disebabkan oleh Hexamitodera semivelutina Hell. Di Desa Rerer Kabupaten Minahasa. Eugenia, 23(2), 76–81.
Scalvenzi, L., Durofil, A., Cáceres Claros, C., Pérez Martínez, A., Guardado Yordi, E., Manfredini, S., Baldini, E., Vertuani, S., & Radice, M. (2024). Unleashing nature’s pesticide: A Systematic review of Schinus molle essential oil’s biopesticidal potential. Sustainability, 16(23), 10444. https://doi.org/10.3390/su162310444
Shannag, H. K., Capinera, J. L., & Freihat, N. M. (2015). Effects of neem-based insecticides on consumption and utilization of food in larvae of Spodoptera eridania (Lepidoptera: Noctuidae). Journal of Insect Science, 15(1), 152. https://doi.org/10.1093/jisesa/iev134
Sujak, Subiyakto, & Sunarto, D. A. (2020). Effectiveness of Botanical Insecticide Mixture of Neem Seed Extract and Citronella Oil Against Cotton Bollworm (Helicoverpa armigera Hubner) and Armyworm (Spodoptera litura Fabricius). Proceedings of the International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019). International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019), Kuta, Bali, Indonesia. https://doi.org/10.2991/absr.k.200513.031
Tak, J.-H., & Isman, M. B. (2016). Metabolism of citral, the major constituent of lemongrass oil, in the cabbage looper, Trichoplusia ni, and effects of enzyme inhibitors on toxicity and metabolism. Pesticide Biochemistry and Physiology, 133, 20–25. https://doi.org/10.1016/j.pestbp.2016.03.009
Tarore, D., Manueke, J., Assa, B. H., & Mamahit, J. E. M. (2020). Use of microbial insecticides in control stem borer (Hexamithodera semivelutina Hell.) on clove plant (Eugenia aromatica O.K.). International Journal of ChemTech Research, 13(3), 83–90. https://doi.org/10.20902/IJCTR.2019.130303
Telaumbanua, M., Savitri, E. A., Shofi, A. B., Suharyatun, S., Wisnu, F. K., & Haryanto, A. (2021). Plant-based pesticide using citronella (Cymbopogon nardus L.) extract to control insect pests on rice plants. IOP Conference Series: Earth and Environmental Science, 739(1), 012071. https://doi.org/10.1088/1755-1315/739/1/012071
Thu, P. Q., Quang, D. N., Chi, N. M., Hung, T. X., Binh, L. V., & Dell, B. (2021). New and emerging insect pest and disease threats to forest plantations in Vietnam. Forests, 12(10), 1301. https://doi.org/10.3390/f12101301
Tumewan, F. N., Watung, J., Lengkong, M., & Kristiningtyas, D. R. (2020). Kajian pengendalian hama penggerek batang cengkeh (Hexamitodera semivelutina Hell.) menggunakan metabolit sekunder jamur Metarhizium dan Beauveria dengan metode infus akar. COCOS, 12(1), 1–7.
Wahedi, J., David, D., Danba, E., Yisa, S., & Zakariya, R. (2016). Yield Performance of Maize Treated with Neem Seed Extracts against Stem Borers. American Journal of Experimental Agriculture, 12(6), 1–8. https://doi.org/10.9734/AJEA/2016/26485
Wani, S. H., Choudhary, M., Barmukh, R., Bagaria, P. K., Samantara, K., Razzaq, A., Jaba, J., Ba, M. N., & Varshney, R. K. (2022). Molecular mechanisms, genetic mapping, and genome editing for insect pest resistance in field crops. Theoretical and Applied Genetics, 135(11), 3875–3895. https://doi.org/10.1007/s00122-022-04060-9
Widodo, Supriyanto, Prasetyo, G. D., Wiyono, S., & Triwidodo, H. (2020). Recent condition of clove death syndrome disease in Indonesia: Epidemiology and causal agents. IOP Conference Series: Earth and Environmental Science, 418(1), 012082. https://doi.org/10.1088/1755-1315/418/1/012082
Wiratno, Maris, P., Sari, M. P., & Wahyuno, T. E. (2020). Toxicity of entomopathogenic fungi, Beauveria bassiana, and clove oil-based pesticide to the main pests of black pepper. IOP Conference Series: Earth and Environmental Science, 418(1), 012055. https://doi.org/10.1088/1755-1315/418/1/012055
Xu, L., Zhao, J., Xu, D., Xu, G., Peng, Y., & Zhang, Y. (2024). New insights into chlorantraniliprole metabolic resistance mechanisms mediated by the striped rice borer cytochrome P450 monooxygenases: A case study of metabolic differences. Science of The Total Environment, 912, 169229. https://doi.org/10.1016/j.scitotenv.2023.169229
Zhao, X., Xu, X., Wang, X., Yin, Y., Li, M., Wu, Y., Liu, Y., Cheng, Q., Gong, C., & Shen, L. (2021). Mechanisms for multiple resistances in field populations of rice stem borer, Chilo suppressalis (Lepidoptera: Crambidae) from Sichuan Province, China. Pesticide Biochemistry and Physiology, 171, 104720. https://doi.org/10.1016/j.pestbp.2020.104720
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